Atopic dermatitis (AD) is a chronic, complex allergic inflammatory skin disease that affects 10-20% of the population, characterised by a periodic flare of the symptoms which include red and itchy skin, swollen and cracked.Initial immune response begins with the T helper (Th)2 response which then shifts to Th1 in the chronic phase.Primary Keratinocyte monocultureHuman immortalized keratinocytes monoculture Primary/immortalised keratinocytes cocultured with immune cells Involves stimulation of human epidermal keratinocytes (NHEK) using a cocktail of proinflammatory agents, including Poly(I:C) and (TNF)-a (Th1cytokine) Or Poly(I:C), IL-4 and IL-13 (Th2 cytokines) to induce AD disease phenotype Measure expression levels of Eotaxin, IL1α, IL4, TNFα, GM-CSF, IL1β, IL8, TSLP, IL18, IFNα2, IFNβ1, MIP1α, RANTES, MIP3α, MDC, CCL27, involucrin and filaggrin to test the efficacy of your test compoundsExplore TLR3/RLRs signaling: NFkB, IRF3 translocation/ phosphorylation; JAK/STAT, PI3K signalling to understand mechanism of action