Atopic dermatitis (AD) is a chronic, complex allergic inflammatory skin disease that affects 10-20% of the population, characterised by a periodic flare of the symptoms which include red and itchy skin, swollen and cracked.Initial immune response begins with the T helper (Th)2 response which then shifts to Th1 in the chronic phase.Primary Keratinocyte monocultureHuman immortalized keratinocytes monoculture Primary/immortalised keratinocytes cocultured with immune cells Involves stimulation of human epidermal keratinocytes (NHEK) using a cocktail of proinflammatory agents, including Poly(I:C)
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